Name: P22555_1003_P18163_V10A21-050_D1_p
Instrument: Illumina NovaSeq 6000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: Oligo-dT
Layout: PAIRED
Construction protocol: L. sclopetarius adult female spiders were collected in the wild in a small habitat in Uppsala, Sweden. Taxonomic identity of the spider was verified by the Museum of Natural History, Stockholm, Sweden. The spiders were kept in big containers that allowed them to spin webs. They were fed with meal worms or Drosophila flies weekly and watered daily. Whole opisthosomas of spiders were flash-frozen in OCT medium on an isopentane-dry ice bath and samples were stored at –80°C until use. The samples were sectioned in a cryotome (10 μm) with knife temperature set at –23°C and sample holder temperature at –10°C. Eight sections from five individual spiders were carefully mounted on the capture areas (6.5 x 6.5 mm) of the Visium spatial slide (10X genomics) and permeabilized for 30 minutes following the 10X Visium spatial tissue optimization protocol. The libraries were constructed according to Visium spatial gene expression protocol (10X genomics). For cDNA amplification 13-16 PCR cycles were performed and for the indexing 13 PCR cycles were used.